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Production of (S)-styrene oxide using styrene oxide isomerase negative mutant of Pseudomonas putida SN1

Paper ID Volume ID Publish Year Pages File Format Full-Text
18347 42719 2006 6 PDF Available
Title
Production of (S)-styrene oxide using styrene oxide isomerase negative mutant of Pseudomonas putida SN1
Abstract

A styrene-degrading strain, Pseudomonas putida SN1, was engineered to produce enantiopure (S)-styrene oxide. Among four catabolic genes, styABCD, which encode the enzymes responsible for styrene degradation, styC was disrupted by homologous recombination to remove styrene oxide isomerase activity that converts styrene oxide to phenylacetaldehyde. The styC-negative mutant could not grow on styrene or styrene oxide, and it accumulated enantiopure (S)-styrene when incubated with styrene. When the reaction was conducted with citrate as a carbon source, the mutant exhibited a high whole-cell activity of styrene oxide formation (170 U/g dcw) that was similar to the styrene-degradation activity of the wild-type strain. In a 3 L bioreactor experiment employing a water–bis(2-ethylhexyl)phthalate two-phase system, in 13 h the mutant could accumulate approximately 150 mM (S)-styrene oxide at an enantiomeric excess greater than 98% in the organic phase.

Keywords
(S)-Styrene oxide; Styrene monooxygenase; Styrene oxide isomerase; Pseudomonas putida SN1; styC-deleted mutant
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Production of (S)-styrene oxide using styrene oxide isomerase negative mutant of Pseudomonas putida SN1
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 6, 3 October 2006, Pages 1264–1269
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us