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Characterization of Thermoanaerobacter cyclomaltodextrin glucanotransferase immobilized on glyoxyl-agarose

Paper ID Volume ID Publish Year Pages File Format Full-Text
18348 42719 2006 9 PDF Available
Title
Characterization of Thermoanaerobacter cyclomaltodextrin glucanotransferase immobilized on glyoxyl-agarose
Abstract

This paper presents the immobilization of the Thermoanaerobacter cyclomaltodextrin glucanotransferase (CGTase) enzyme into cross-linked 6% agarose beads activated by high density of linear aldehyde groups (glyoxyl-agarose) that allow the establishment of multi-attachment enzyme-support bonds. The immobilization conditions were 25 °C, pH 10 and 5 h of contact time. The immobilization yield was almost 100% and the activity recovery was ca. 32%. The biocatalyst at 85 °C was capable of producing cyclodextrins (CDs) from dextrin or soluble starch (both at 1% (w/v)) at a greater rate than the soluble enzyme. In addition, the biocatalyst maintained 90% of its initial activity after 5 h at 85 °C. The maximum conversion of dextrin to β-CD and γ-CD (total mass of produced CDs/substrate initial mass × 100) was 29% both for the soluble and immobilized enzymes. Using starch as substrate the maximum starch conversion to β-CD and γ-CD was 29% and 38%, for the immobilized and soluble enzyme, respectively. The β-CD selectivity yield [mass of β-CD produced/(mass of β-CD produced + mass of γ-CD produced) × 100] increased from 67.9% for the free enzyme to 85.4% for the immobilized CGTase.

Keywords
Cyclodextrin; Cyclomaltodextrin; Starch; Glyoxyl-agarose; Cyclomaltodextrin glucanotransferase; Cyclodextrin glycosyltransferase
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Characterization of Thermoanaerobacter cyclomaltodextrin glucanotransferase immobilized on glyoxyl-agarose
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 6, 3 October 2006, Pages 1270–1278
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us