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Regulation of CCR in the γ-CGTase production from Bacillus macorous by the specific cell growth rate control

Paper ID Volume ID Publish Year Pages File Format Full-Text
18349 42719 2006 7 PDF Available
Title
Regulation of CCR in the γ-CGTase production from Bacillus macorous by the specific cell growth rate control
Abstract

Malto-oligosaccharides were products of the degradation of corn starch in the γ-CGTase production by Bacillus macorous WSH02-06, and catabolically repressed the synthesis of γ-CGTase. The investigation of the enzyme production at different agitation speeds, pH-stat values and temperatures revealed that the amount of malto-oligosaccharides accumulated in cultivation was in direct connection with the specific cell growth rate. When the maximal specific growth rate was higher than 0.2 h−1, the γ-CGTase yield decreased. Thus, the nutrients were supplied in the exponential feeding mode for limiting the maximal specific growth rate and the γ-CGTase yield was promoted to the highest value of 2565 U mL−1 that was 6.88-fold higher than that achieved in the batch fermentation.

Keywords
γ-CGTase production; Bacillus macorous; CCR; Specific growth rate; Exponential feeding
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Regulation of CCR in the γ-CGTase production from Bacillus macorous by the specific cell growth rate control
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 6, 3 October 2006, Pages 1279–1285
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us