fulltext.study @t Gmail

Pore diffusion studies with immobilized glucose oxidase plus catalase membranes

Paper ID Volume ID Publish Year Pages File Format Full-Text
18354 42719 2006 6 PDF Available
Title
Pore diffusion studies with immobilized glucose oxidase plus catalase membranes
Abstract

Glucose oxidase (GOD) and catalase (CAT) were chemically immobilized onto three types of ultrafiltration polyacrylonitrile membranes with increase pore sizes (PAN 1–PAN 3) and one microfiltration polyamide membrane. The initial membranes were modified to generate amino and amide groups on their surface, necessary for the covalent immobilization of the enzymes. The diffusivity parameter of glucose through the initial and modified membranes was determined. It was found out that the glucose diffusion, increased with the increase of the pore size. The slight increase of glucose diffusion parameters was observed for the modified membranes in comparison with the initial membranes. The diffusivity of glucose through the inactivated enzyme membranes was determined also. A minor decrease in the values of these parameters with respect to glucose was observed for the enzyme PAN 2 membrane, while for the PAN 3 and PA enzyme membrane there was two-fold decrease in the obtained parameter values (compared to the initial and modified membranes). There was no significant change in the values of the indicated parameters with respect to H2O2 for different modified and enzyme membranes.The mass transfer coefficients (KL), thickness of boundary layer (δ), Sherwood number (Sh) and Thiele modulus (ϕ) as well as their influence on the efficiency of the enzyme couple GOD–CAT immobilized onto different pore size polymer membranes were also studied. The final results showed that the two immobilized systems with PAN 3 and PA membranes, as carriers were effective as compared to PAN 1 and PAN 2 membranes.

Keywords
Membranes; Enzymes; Immobilization; Diffusion; Effectiveness factor
First Page Preview
Pore diffusion studies with immobilized glucose oxidase plus catalase membranes
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 6, 3 October 2006, Pages 1313–1318
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us