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The importance of 293 cell cycle phase on adenovirus vector production

Paper ID Volume ID Publish Year Pages File Format Full-Text
18356 42719 2006 5 PDF Available
Title
The importance of 293 cell cycle phase on adenovirus vector production
Abstract

One of the major restrictions on the production of adenoviral vectors (AdV) is the so-called “cell density effect”, i.e., a drop in cell-specific AdV productivity concomitant with increased cell concentration at infection (CCI) above 1 × 106 cell/ml. Although this “cell density effect” has been considered by several authors as a consequence of nutrient depletion and/or accumulation of byproducts, this study shows that the cell fraction at the S phase of the cell cycle may also play a role on AdV production at high CCI in 293 batch cultures. By infecting cells obtained from different growth stages at cell densities of 0.7 (lag), 1.6 (middle exponential), 3.3 (late exponential) and 5.0 × 106 (stationary) cell/ml, consisting of a descending proportion of cells in the S phase of the cell cycle in equalized cell density condition, it is shown that cell-specific AdV productivity is dependent on the cell cycle phase at infection at both the optimal CCI (1 × 106 cell/ml) and high CCI (4 × 106 cell/ml). In particular, under the equalized cell density conditions, the infection of cells with an S phase proportion of 50% at the high CCI gave the higher AdV volumetric productivity. Moreover, the lowest cell-specific AdV productivity was obtained with the lowest S phase proportion of 28% for both CCIs. These results indicate that a decrease in the proportion of cells at S phase at the time of infection also contributes to a decreased cell-specific AdV productivity at high cell densities.

Keywords
Adenovirus vector production; Cell density effect; Cell cycle; 293 Cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 6, 3 October 2006, Pages 1328–1332
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us