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Application of the response surface methodology (RSM) for optimizing the production of volatile organic compounds (VOCs) by Trichosporon moniliiforme

Paper ID Volume ID Publish Year Pages File Format Full-Text
18358 42719 2006 6 PDF Available
Title
Application of the response surface methodology (RSM) for optimizing the production of volatile organic compounds (VOCs) by Trichosporon moniliiforme
Abstract

Response surface methodology (RSM) was applied in optimizing the production of volatile organic compounds (VOCs) by shake-flask cultures of a selected Trichosporon moniliiforme strain (DBVPG 4665). A factorial experiment (23) was designed by setting three parameters at two levels: X1 = incubation time (IT = 72–168 h); X2 = pH (4–7); X3 = l-methionine concentration (l-MET = 5–20 g l−1). On the basis of the results of factorial design, X2 (pH) and X3 (l-MET) were optimized by performing a central composite design (CCD). Second-order polynomial models and response surfaces were obtained for total VOC concentration (VOCl) and for the percentage of dimethyl disulphide (DMDS)/total VOCs (%DMDS). A range of variation of X2 and X3 able to maximize simultaneously both responses has been found. Average final values of responses (VOCl = 4.9 ± 1.3 mg l−1; %DMDS = 97.6 ± 1.1%) were about 532 and 133% of data of central point and were in good agreement with calculated values, thus confirming the reliability of our empirical model in optimizing VOCs production by T. moniliiforme

Keywords
Yeasts; VOCs; Dimethyl disulphide; Response surface methodology
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Application of the response surface methodology (RSM) for optimizing the production of volatile organic compounds (VOCs) by Trichosporon moniliiforme
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 6, 3 October 2006, Pages 1341–1346
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us