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Screening methods for synthetic activity of lipases

Paper ID Volume ID Publish Year Pages File Format Full-Text
18366 42720 2007 4 PDF Available
Title
Screening methods for synthetic activity of lipases
Abstract

In order to optimize synthetic biocatalytic transformations catalyzed by lipases, a large number of enzyme preparations must be tested. Moreover, to design an enzyme for an industrial application it is generally necessary to realize enzyme evolution to improve enzyme performances, for instance thermostability, activity or selectivity. The crucial step is then the screening of the optimal catalyst. Because hydrolytic and synthetic activities are not always correlated, specific screening methods for synthesis activity of lipases are needed. In this work, we report three sensitive and specific screening methods to measure synthetic activity of lipases in vivo on an agar plate and in vitro, directly from culture broth.

Keywords
Lipase; Screening; Synthetic activity
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Screening methods for synthetic activity of lipases
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 3, 5 February 2007, Pages 390–393
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us