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Amperometric immunosensor for the detection of anti-West Nile virus IgG using a photoactive copolymer

Paper ID Volume ID Publish Year Pages File Format Full-Text
18368 42720 2007 6 PDF Available
Title
Amperometric immunosensor for the detection of anti-West Nile virus IgG using a photoactive copolymer
Abstract

A polymeric film consisting of two distinct monomers such as pyrrole–benzophenone and tris(bipyridine pyrrole) ruthenium (II) units was electrogenerated and used for the photochemical grafting of T7 bacteriophages displaying a specific West Nile virus (WNV) epitope. The resulting electrodes were applied to the amperometric detection of WNV antibody at 0 V in aqueous electrolyte via secondary peroxidase-labeled antibody, hydrogen peroxide and hydroquinone as the enzyme substrate. A calibration curve based on different WNV–antibody dilutions ranging from 10 to 106 was created with a relatively short response time of 30 s after substrate injection.

Keywords
West Nile virus antibodies; Bacteriophage; Immunosensor; Polypyrrole; Benzophenone
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Amperometric immunosensor for the detection of anti-West Nile virus IgG using a photoactive copolymer
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 3, 5 February 2007, Pages 403–408
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us