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Characterization of news proteolytic enzymes from ripe fruits of Bromelia antiacantha Bertol. (Bromeliaceae)

Paper ID Volume ID Publish Year Pages File Format Full-Text
18369 42720 2007 5 PDF Available
Title
Characterization of news proteolytic enzymes from ripe fruits of Bromelia antiacantha Bertol. (Bromeliaceae)
Abstract

Crude extracts were partially purified by organic solvents fractionation: best results (96% of proteins, 91% of total caseinolytic activity) were obtained by adding four volumes of cold acetone to the crude extract. This preparation (redissolved acetone precipitate, RAP) showed maximum activity (>80%) at pH 5–9, and exhibited high thermal stability (>90% of residual activity after heating for 60 min at 60 °C). The enzyme was completely inhibited by E-64 trans-epoxysuccinyl-leucyl-amido(4-guanidino)-butane and iodoacetic acid and activated by the addition of cysteine or β-mercaptoethanol; these results strongly suggest that the isolated protease should be included within the cysteine group, as all the other studied proteases belonging to the family Bromeliaceae. IEF-zymogram of RAP showed five bands (pI 7.3 to <9.3), most of them proteolytically actives, but only three of which (pI 7.6, 8.2 and 8.8) proved to be important. Ion exchange chromatography in DEAE-Sephadex, was selected to separate this active bands.

Keywords
Bromelia antiacantha; Fruit proteinases; Cysteine protease
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Characterization of news proteolytic enzymes from ripe fruits of Bromelia antiacantha Bertol. (Bromeliaceae)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 3, 5 February 2007, Pages 409–413
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us