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New biochemical methods for production of Nα-benzyloxycarbonyl-d,l-aminoadipic-δ-semialdehyde and Nα-benzyloxycarbonyl-d,l-aminoadipic acid

Paper ID Volume ID Publish Year Pages File Format Full-Text
18373 42720 2007 4 PDF Available
Title
New biochemical methods for production of Nα-benzyloxycarbonyl-d,l-aminoadipic-δ-semialdehyde and Nα-benzyloxycarbonyl-d,l-aminoadipic acid
Abstract

New biochemical methods for the production of Nα-benzyloxycarbonyl-l-aminoadipate δ-semialdehyde (Nα-Z-l-AASA), Nα-Z-d-AASA, Nα-Z-l-aminoadipic acid (Nα-Z-l-AAA), Nα-Z-d-AAA were developed using cells of Rhodococcus sp. AIU Z-35-1. When the cells harvested after 1 day of cultivation were incubated with 100 mM Nα-Z-l-lysine at pH 5.0 for 1 day at 30 °C or at pH 7.0 for 2 days at 10 °C, more than 95 mM Nα-Z-l-AASA was produced. Using the cells harvested after 3 days of cultivation, Nα-Z-l-AAA was efficiently produced by incubating at pH 7.0 for 4 days at 30 °C. Similar conversion yields of Nα-Z-d-AASA and Nα-Z-d-AAA were also obtained under the same conditions. Thus, the microbial methods proposed here were superior to the chemical and other biochemical methods in simplicity, reaction rate, and yield.

Keywords
Nα-Z-l-lysine; Nα-Z-d-lysine; Nα-Z-l-aminoadipate δ-semialdehyde; Nα-Z-d-aminoadipate δ-semialdehyde; Nα-Z-l-aminoadipic acid; Nα-Z-d-aminoadipic acid
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New biochemical methods for production of Nα-benzyloxycarbonyl-d,l-aminoadipic-δ-semialdehyde and Nα-benzyloxycarbonyl-d,l-aminoadipic acid
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 3, 5 February 2007, Pages 433–436
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us