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Stability of immobilized Candida antarctica lipase B during chemo-enzymatic epoxidation of fatty acids

Paper ID Volume ID Publish Year Pages File Format Full-Text
18376 42720 2007 5 PDF Available
Title
Stability of immobilized Candida antarctica lipase B during chemo-enzymatic epoxidation of fatty acids
Abstract

The parameters affecting the lipase activity and operational lifetime during chemo-enzymatic epoxidation of fatty acids were investigated. Immobilized Candida antarctica lipase B (Novozym® 435) was incubated in the presence of various reaction components (i.e. toluene, water, H2O2, oleic acid, perpalmitic acid, and epoxystearic acid, respectively) at temperatures between 20 and 60 °C followed by measurement of residual enzyme activity. Epoxystearic acid was shown to slightly inactivate the enzyme at 50 °C, while oleic acid and perpalmitic acid did not. No deactivation of the enzyme was observed in presence of toluene/water mixture within 48 h at 20–60 °C. In the presence of 6–12 M hydrogen peroxide, the enzyme was rather stable at 20 °C, while at 60 °C the enzyme lost activity rapidly, with the rate of deactivation increasing with increasing hydrogen peroxide concentration. These results imply that temperature control and careful dosage of hydrogen peroxide would be essential in an industrial chemo-enzymatic process.

Keywords
Epoxidation; Lipase deactivation; Solvent-free process; Hydrogen peroxide; Peracid; Oxidation
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Stability of immobilized Candida antarctica lipase B during chemo-enzymatic epoxidation of fatty acids
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 3, 5 February 2007, Pages 447–451
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us