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Preparation of a crude enzymatic from Bacillus licheniformes E-44 and its evaluation in the hydrolysis of Saccharomyces cerevisiae cell walls

Paper ID Volume ID Publish Year Pages File Format Full-Text
18377 42720 2007 4 PDF Available
Title
Preparation of a crude enzymatic from Bacillus licheniformes E-44 and its evaluation in the hydrolysis of Saccharomyces cerevisiae cell walls
Abstract

The microbial growth, endospores production and protease activity was investigated during growth of Bacillus licheniformes E-44 on nutrient broth medium at 40 °C. The total number of bacterial cells (8.8 × 1010 UFC/ml), endospores production (8 × 107 UFC/ml) and protease activity (17.8 Anson U/ml) were maximal after 20 h of cultivation. The hydrolytic activity of the produced crude enzymatic preparation was assessed on residual distillery's yeast cream for obtaining a Saccharomyces cerevisiae hydrolysate (SCH). An increase of the concentration of gluco- and manno-oligosaccharides and total reducing sugars was observed during the production of SCH. The highest concentrations of gluco- and manno-oligosaccharides and total reducing sugars were achieved after 16 and 20 h, respectively.

Keywords
Bacillus licheniformes; Hydrolytic enzymes; Saccharomyces cerevisiae; Gluco-oligosaccharides; Manno-oligosaccharides
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Preparation of a crude enzymatic from Bacillus licheniformes E-44 and its evaluation in the hydrolysis of Saccharomyces cerevisiae cell walls
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 3, 5 February 2007, Pages 452–455
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
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Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us