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Colorimetric assay of ethanol using alcohol dehydrogenase from dry baker's yeast

Paper ID Volume ID Publish Year Pages File Format Full-Text
18380 42720 2007 5 PDF Available
Title
Colorimetric assay of ethanol using alcohol dehydrogenase from dry baker's yeast
Abstract

Alcohol dehydrogenases (ADHs) are oxidoreductases present in animal tissues, plants, and microorganisms. These enzymes attract major scientific interest for the evolutionary perspectives, afforded by their wide occurrence in nature, and for their use in synthesis, thanks to their broad substrate specificity and stereoselectivity. In the present study, the standardization of the activity of the alcohol dehydrogenase from baker's yeast was accomplished, and the pH and temperature stability showed, that the enzyme presented a high stability to pH 6.0–7.0 and the thermal stability were completely maintained up to 50 °C during 1 h. The assays of ethanol (detection range 1–5 mM or 4.6 × 10−2 to 23.0 × 10−2 g/L) in different samples in alcoholic beverages, presented a maximum deviation of only 7.2%. The standard curve and the analytic curve of this method meet the conditions of precision, sensitivity, simplicity, and low cost, required for a useable analytical method.

Keywords
Alcohol dehydrogenase; Colorimetric assay of ethanol; Baker's yeast
First Page Preview
Colorimetric assay of ethanol using alcohol dehydrogenase from dry baker's yeast
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 3, 5 February 2007, Pages 466–470
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering