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Production and down-stream processing of an extracellular lipase from the yeast Yarrowia lipolytica

Paper ID Volume ID Publish Year Pages File Format Full-Text
18391 42721 2006 4 PDF Available
Title
Production and down-stream processing of an extracellular lipase from the yeast Yarrowia lipolytica
Abstract

Lipase constitutes an interesting class of enzyme with many biotechnological applications. However, the development of a fruitful process must be set up to obtained a product compatible with the industrial and commercial needs. Here, we report the development of such a process for the extracellular lipase secreted by the yeast Yarrowia lipolytica. The enzyme production, carried out in a 2000 L bioreactor, led to a lipase activity of approximately 1100 U mL−1 after 53 h of fermentation. The post-culture treatment, consisting of a centrifugation, a filtration and an ultra-filtration steps, led to 15-fold volume reduction and a 8-fold increased of the lipase activity. Finally, addition of 12% of milk powder and 3% of gum arabic before spray-drying dehydration led to a stable powder with a lipolytic activity of 37,500 U g D.W.−1.

Keywords
Lipase; Bioreactor; Down-stream process; Yarrowia lipolytica
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Production and down-stream processing of an extracellular lipase from the yeast Yarrowia lipolytica
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 38, Issue 6, 1 April 2006, Pages 756–759
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us