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Production of isoamyl acetate with immobilized Staphylococcus simulans lipase in a solvent-free system

Paper ID Volume ID Publish Year Pages File Format Full-Text
18396 42721 2006 7 PDF Available
Title
Production of isoamyl acetate with immobilized Staphylococcus simulans lipase in a solvent-free system
Abstract

A non-commercial immobilized lipase preparation from Staphylococcus simulans was used to catalyze the esterification reaction between acetic acid and isoamyl alcohol to synthetize isoamyl acetate (banana flavour) in pure substrate conditions (without addition of any organic solvent). The effects of various reaction parameters such as the amount of lipase, the molar ratio of acetic acid as compared to isoamyl alcohol and the amount of initially added water were investigated. The optimum conditions for achieving 64% conversion during 8 h of incubation are 60 IU of immobilized lipase, an acid/alcohol of 2 molar ratio and an initial addition of water of 10% (w/w). No significant loss of the immobilized lipase activity was observed after four cycles of use of the immobilized enzymatic preparation.

Keywords
Isoamyl acetate; Esterification; Solvent-free system; Immobilized lipase
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Production of isoamyl acetate with immobilized Staphylococcus simulans lipase in a solvent-free system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 38, Issue 6, 1 April 2006, Pages 788–794
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us