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Large scale production of d-allose from d-psicose using continuous bioreactor and separation system

Paper ID Volume ID Publish Year Pages File Format Full-Text
18404 42721 2006 5 PDF Available
Title
Large scale production of d-allose from d-psicose using continuous bioreactor and separation system
Abstract

l-Rhamnose isomerase (l-RhI) from Pseudomonas stutzeri LL172 can convert d-psicose to d-allose. Partially purified recombinant l-RhI from Escherichia coli was immobilized on BCW-2510 Chitopearl beads and utilized to produce d-allose. Total 20,000 units of immobilized enzyme converted d-psicose to d-allose without remarkable decrease in the enzyme activity over 17 days. When 50% d-psicose (w/w) was applied to a column with a flow rate of 0.8 ml/min at 42 °C, approximately 30% d-psicose was isomerized to d-allose for 17 days. However, by reducing the flow rate to 0.4 ml/min after 17 days, d-allose was transformed at the same rate for 13 days. The total of 27 l reaction mixture was separated by Simulated-Moving-Bed Chromatograph system. Approximately 2.2 l/d of 50% (w/w) reaction mixture was separated continuously. After separation, d-allose and d-psicose fractions were 3 l of approximately 10% (w/w) with 95% purity and 10 l of approximately 8% (w/w) with 95% purity per day, respectively. The separated d-allose solution was concentrated up to about 50% and crystallized gradually by being kept at room temperature. Crystals of d-allose were separated from the syrup by filtration and 1.65 kg crystals of 100% purity were obtained. The d-allose crystal yield from the d-psicose substrate was approximately 10%.

Keywords
Rare sugars; d-Allose; l-Rhamnose isomerase; Simulated-Moving-Bed Chromatograph
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Large scale production of d-allose from d-psicose using continuous bioreactor and separation system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 38, Issue 6, 1 April 2006, Pages 855–859
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us