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Liquid–liquid extraction of commercial and biosynthesized nisin by aqueous two-phase micellar systems

Paper ID Volume ID Publish Year Pages File Format Full-Text
18410 42722 2008 6 PDF Available
Title
Liquid–liquid extraction of commercial and biosynthesized nisin by aqueous two-phase micellar systems
Abstract

Nisin is a natural additive for conservation of food, and can also be used as a therapeutic agent. Nisin inhibits the outgrowth of spores, the growth of a variety of Gram-positive and Gram-negative bacteria. In this paper we present a potentially scalable and cost-effective way to purify commercial and biosynthesized in bioreactor nisin, including simultaneously removal of impurities and contaminants, increasing nisin activity. Aqueous two-phase micellar systems (ATPMS) are considered promising for bioseparation and purification purposes. Triton X-114 was chosen as the as phase-forming surfactant because it is relatively mild to proteins and it also forms two coexisting phases within a convenient temperature range. Nisin activity was determined by the agar diffusion assay utilizing Lactobacillus sake as a sensitive indicator microorganism. Results indicated that nisin partitions preferentially to the micelle rich-phase, despite the surfactant concentration tested, and its antimicrobial activity increases. The successful implementation of this peptide partitioning, from a suspension containing other compounds, represents an important step towards developing a separation method for nisin, and more generally, for other biomolecules of interest.

Keywords
Nisin; Aqueous two-phase micellar system; Cloud-point extraction; Triton X-114; Protein purification; Concentration
First Page Preview
Liquid–liquid extraction of commercial and biosynthesized nisin by aqueous two-phase micellar systems
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 42, Issue 2, January 2008, Pages 107–112
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering