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Cloning and characterization of GTP-binding proteins of Mycobacterium tuberculosis H37Rv

Paper ID Volume ID Publish Year Pages File Format Full-Text
18414 42722 2008 7 PDF Available
Title
Cloning and characterization of GTP-binding proteins of Mycobacterium tuberculosis H37Rv
Abstract

GTP-binding proteins (G-proteins) are highly conserved signaling molecules that participate in cellular signaling and bacterial pathogenesis by regulating the activity of cognate GTPases. However, the exact role of G-proteins in the pathogenesis of Mycobacterium tuberculosis is poorly understood. The complete genome sequence of M. tuberculosis H37Rv, suggests the presence of several homologs of bacterial G-proteins. In the present study, three G-proteins, Era, Obg and LepA of M. tuberculosis H37Rv were cloned and expressed in Escherichia coli. Purified proteins showed GTP-binding and hydrolyzing activities. A point mutation in the conserved GTP-binding motif, AspXXGly (Asp to Ala) in Era (Asp-258) and Obg (Asp-212) proteins resulted in the loss of the associated activities, confirming that known key residues in well-established G-proteins are also conserved in mycobacterial homologs. This study confirms that Era, Obg and LepA of M. tuberculosis H37Rv possess GTPase activity and provide a platform to understand the physiological significance of these proteins in associated pathogenesis.

Keywords
Mycobacteria; Tuberculosis; Era; Obg; LepA; GTPase; G-proteins
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Cloning and characterization of GTP-binding proteins of Mycobacterium tuberculosis H37Rv
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 42, Issue 2, January 2008, Pages 138–144
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us