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Effects of cultivation conditions on the production of natamycin with Streptomyces gilvosporeus LK-196

Paper ID Volume ID Publish Year Pages File Format Full-Text
18415 42722 2008 6 PDF Available
Title
Effects of cultivation conditions on the production of natamycin with Streptomyces gilvosporeus LK-196
Abstract

Natamycin is a very attractive antifungal agent with wide applications in medical and food industries. In order to improve the productivity of natamycin, the effects of cultivation conditions were investigated with Streptomyces gilvosporeus LK-196 in the shake flasks and 30-L fermentors. The results showed that dissolved oxygen and shear force would affluence the biosynthesis of natamycin significantly. The high concentration of natamycin (2.03 g/L) was achieved under the suitable culture conditions in the shake flask scale. Further investigations in 30-L fermentors showed that the optimal pH was controlled at 6.0 during the whole bioprocess, and the dissolved oxygen level should be more than 30% by adjusting the aeration and agitation rates for high production of natamycin. Under these optimal conditions the high concentration of natamycin (3.94 g/L) was achieved with Str. gilvosporeus LK-196 in the 30-L fermentor. Finally, the high-level fermentation process was successfully scaled up to 1000-L fermentors and 18,000-L fermentors in the pilot plant.

Keywords
Natamycin; Streptomyces gilvosporeus; Cultivation conditions
First Page Preview
Effects of cultivation conditions on the production of natamycin with Streptomyces gilvosporeus LK-196
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 42, Issue 2, January 2008, Pages 145–150
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering