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High poly(β-hydroxybutyrate) production by Pseudomonas fluorescens A2a5 from inexpensive substrates

Paper ID Volume ID Publish Year Pages File Format Full-Text
18418 42722 2008 6 PDF Available
Title
High poly(β-hydroxybutyrate) production by Pseudomonas fluorescens A2a5 from inexpensive substrates
Abstract

A strain of poly(β-hydroxybutyrate) (PHB)-accumulating bacterium was isolated from the soil in Alaska of USA, identified as Pseudomonas fluorescens, and designated as strain A2a5. The organism grew at temperatures below 30 °C, and accumulated a large amount of granules in its cells when it was cultured in the sugarcane liquor medium. The purified sample from cells was determined as PHB by gas chromatographic and nuclear magnetic resonance analysis of polyesters. The cell density of the culture in shaking bottles reached OD600 = 155 with PHB concentration of 31 g l−1. In 5 l bioreactor, a maximum cell dry weight (CDW) of 32 g l−1 with a PHB concentration of 22 g l−1 was obtained, and the PHB content was up to 70% and productivity was 0.23 g l−1 h−1.

Keywords
Pseudomonas fluorescens A2a5; Poly(β-hydroxybutyrate) (PHB); Sugarcane liquor; Gas chromatography; Nuclear magnetic resonance; Batch fermentation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 42, Issue 2, January 2008, Pages 167–172
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us