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Biphasic culture strategy for enhancing volumetric erythropoietin productivity of Chinese hamster ovary cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
18427 42723 2006 4 PDF Available
Title
Biphasic culture strategy for enhancing volumetric erythropoietin productivity of Chinese hamster ovary cells
Abstract

In the case of Chinese hamster ovary (CHO) cells producing erythropoietin (EPO), an optimal EPO production condition (32.5 °C and pH 7.00) was quite different from an optimal growth condition (37 °C and pH 7.20). When cells were cultivated at the two culture conditions, the EPO production at the optimal EPO production condition was approximately 3.1-fold higher than that at the optimal growth condition. However, reduced cell growth rate at the optimal EPO production condition led to only 1.2-fold increase in the volumetric productivity, compared to the optimal EPO growth condition. A biphasic culture strategy, which is composed of cell growth and EPO production phases, was applied to increase the volumetric EPO productivity. Compared with the monophasic culture at the optimal EPO production condition, a 1.4-fold increase in the volumetric productivity was achieved in the biphasic culture at a shifting time of 2 days post-inoculation. Thus, a biphasic culture strategy was found to be an efficient means to increase the volumetric EPO productivity of rCHO cells with different optimal growth and EPO production conditions.

Keywords
CHO cells; Erythropoietin (EPO); Biphasic culture; Volumetric productivity
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Biphasic culture strategy for enhancing volumetric erythropoietin productivity of Chinese hamster ovary cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 3, 3 July 2006, Pages 362–365
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us