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Response surface methodological approach for optimization of enzymatic synthesis of sorbitan methacrylate

Paper ID Volume ID Publish Year Pages File Format Full-Text
18431 42723 2006 6 PDF Available
Title
Response surface methodological approach for optimization of enzymatic synthesis of sorbitan methacrylate
Abstract

Sorbitan methacrylate was synthesized from sorbitan dehydrated from d-sorbitol using an immobilized lipase. To optimize the enzymatic synthesis of sorbitan methacrylate, response surface methodology was applied to determine the effects of five-level-four-factors and their reciprocal interactions on sorbitan methacrylate biosynthesis. A total of 30 individual experiments were performed, which were designed to study reaction temperature, reaction time, enzyme amount and substrate molar ratio. A statistical model predicted that the highest conversion yield of sorbitan methacrylate was 100%, at the following optimized reaction conditions: a reaction temperature of 43.06 °C, a reaction time of 164.25 min, an enzyme amount of 7.47%, and a substrate molar ratio of 3.98:1. Using these optimal factor values under experimental conditions in four independent replicates, the average conversion yield reached 98.7 ± 1.2% and was well within the value predicted by the model.

Keywords
Immobilized enzyme; Bioconversion; Optimization; Sorbitan; Esterification; Response surface methodology
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Response surface methodological approach for optimization of enzymatic synthesis of sorbitan methacrylate
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 3, 3 July 2006, Pages 381–386
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us