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Performance enhancement of real-time detection of protozoan parasite, Cryptosporidium oocyst by a modified surface plasmon resonance (SPR) biosensor

Paper ID Volume ID Publish Year Pages File Format Full-Text
18432 42723 2006 4 PDF Available
Title
Performance enhancement of real-time detection of protozoan parasite, Cryptosporidium oocyst by a modified surface plasmon resonance (SPR) biosensor
Abstract

Surface plasmon resonance (SPR) biosensor based on mixed self-assembled monolayers (SAMs) was developed for real-time detection of Cryptosporidium oocyst. The flow type SPR biosensor consisting of subsequent binding of antibody and oocyst in flowing buffer system was able to detect Cryptosporidium parvum oocyst in real-time with a detection limit of 1 × 106 oocyst/ml. To enhance the sensitivity of real-time detection, the modified flow type SPR biosensor was developed, which consisted of the immunoreaction step between the biotin-antibody and oocysts followed by the binding step of antibody bound oocysts onto the sensor surface. Not only was the immunoreaction yield with batch immune reaction improved, but the accessibility of analytes to the sensor surface was also enhanced since the binding event for SPR signal generation was exchanged to biotin–streptavidin binding with high affinity. Consequently, the real-time detection limit of 1 × 102 oocyst/ml was obtained in the modified flow type biosensor.

Keywords
Surface plasmon resonance; Cryptosporidium oocyst; Real-time detection; Biosensor
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Performance enhancement of real-time detection of protozoan parasite, Cryptosporidium oocyst by a modified surface plasmon resonance (SPR) biosensor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 3, 3 July 2006, Pages 387–390
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us