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Development of serum-free media for a recombinant CHO cell line producing recombinant antibody

Paper ID Volume ID Publish Year Pages File Format Full-Text
18438 42723 2006 8 PDF Available
Title
Development of serum-free media for a recombinant CHO cell line producing recombinant antibody
Abstract

A serum-free medium (SFM-MDM) without any animal-driven protein was developed for the production of recombinant antibody in suspension cultures of recombinant Chinese hamster ovary cell line, CS*13-1.00. The formulation of SFM-MDM was based upon the Iscove's modified Dulbecco's medium (IMDM), and Long™R3IGF-1 and ferric citrate were supplemented instead of insulin and transferrin. Among various protein hydrolysates, yeast extract was selected as a serum-substituting additive for the improvement cell growth and productivity of recombinant antibody. Through about 3600 h of adaptation period from conventional SFM to SFM-MDM, integral of viable cell density and specific antibody productivity increased from 7.60 × 106 cells d and 19.14 μg (106 cells d)−1 to 7.79 × 106 cells d and 54.04 μg (106 cells d)−1, respectively, resulted in final antibody concentration of 420.98 mg l−1, which was about three times higher than that obtained from commercial medium, 486F. Also, supplementation of various vitamins including biotin, pyridoxal·HCl, and ascorbic acid was observed to enhance specific productivity, which led to high final antibody concentration. These results suggested the fundamental and effective strategy for development of a serum-free medium and to enhance cell growth and production of target proteins.

Keywords
Serum-free medium; Recombinant antibody production; Chinese hamster ovary cell
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Development of serum-free media for a recombinant CHO cell line producing recombinant antibody
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 3, 3 July 2006, Pages 426–433
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Any Questions? feel free to contact us