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Characterization of S-adenosylmethionine synthetase from Streptomyces avermitilis NRRL8165 and its effect on antibiotic production ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
18444 42723 2006 8 PDF Available
Title
Characterization of S-adenosylmethionine synthetase from Streptomyces avermitilis NRRL8165 and its effect on antibiotic production ☆
Abstract

A putative S-adenosylmethionine synthetase (SAM-s) gene from Streptomyces avermitilis NRRL8165 was cloned and characterized. The gene encodes a protein of 402 amino acids with a molecular weight of about 43.5 kDa. Amino acid sequence analysis revealed high sequence identity (84–93%) to known SAM-s proteins from Streptomyces species containing the conserved sequence motifs of SAM-s proteins, such as nanopeptide 276GGGAFSGKD284 for the P-loop for ATP binding site and metal binding site (17D and 289D for Mg2+ and 49G for K+). The purified protein from the transformed Escherichia coli synthesized S-adenosylmethionine (SAM) from ATP and l-methionine in vitro with a Km of 110(±32) and 340(±18) μM and Vmax of 7.9(±0.2) and 21.6(±1.5) μmol/mg/min for l-methionine and ATP, respectively. This result demonstrates that the isolated gene indeed encoded the SAM-s protein. The enzymatic activity of SAM-s protein was optimal at pH 8.0 and 35 °C and required divalent metal ions (Mg2+ or Mn2+) as a cofactor, like SAM-s proteins from other species. When the SAM-s was overexpressed in S. avermitilis, a wild type producer of avermectin, and Streptomyces peucetius, a wild type producer of doxorubicin, the production of antibiotics significantly increased by 2-fold for avermectin and 3.5-fold for doxorubicin, respectively, compared with the respective wild type strain.

Keywords
Streptomyces avermitilis; S-Adenosylmethionine synthetase; Avermectin; Secondary metabolite
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Characterization of S-adenosylmethionine synthetase from Streptomyces avermitilis NRRL8165 and its effect on antibiotic production ☆
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 3, 3 July 2006, Pages 466–473
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us