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Production of soluble β-glucan from the cell wall of Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
18449 42723 2006 5 PDF Available
Title
Production of soluble β-glucan from the cell wall of Saccharomyces cerevisiae
Abstract

β-Glucan is a major component of the yeast cell wall, and plays an important role in the immune system and skin protection. The composition and structure of the yeast cell wall vary with species. The yeast cell wall is also affected by the composition of media and the culture conditions. After cultivating Saccharomyces cerevisiae, the cell wall fraction was prepared by cell lysis using a homogenizer. The crude cell wall extracts were purified to the soluble β-glucan by ethanol precipitation followed by DEAE ion-exchange chromatography, ConA chromatography, and freeze-drying. In batch cultivation of S. cerevisiae, the soluble β-glucan content and the resistance of the cell wall to β-glucanase was highest at early stationary phase. In continuous cultivation of S. cerevisiae, the soluble β-glucan content and the resistance of cell wall to β-glucanase were higher at lower dilution rate. Based on the results obtained from batch and continuous cultivation of S. cerevisiae, fed-batch cultivation was chosen for the mass production of soluble β-glucan. When S. cerevisiae was grown on 1.5-fold concentrated Dw medium and 15 g of glucose 50 ml−1 solution was fed intermittently, 0.13 g β-glucan g−1 dried cell was obtained, and the productivity of β-glucan was 0.095 g β-glucan l−1 culture broth h−1.

Keywords
β-Glucan; β-Glucanase; Saccharomyces cerevisiae; Cell wall; Fermentation
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Production of soluble β-glucan from the cell wall of Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 3, 3 July 2006, Pages 496–500
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us