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Production, structure elucidation and anticancer properties of sophorolipid from Wickerhamiella domercqiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
18450 42723 2006 6 PDF Available
Title
Production, structure elucidation and anticancer properties of sophorolipid from Wickerhamiella domercqiae
Abstract

A yeast strain Y2A producing a large amount of biosurfactants was isolated from oil-containing wastewater and identified as Wickerhamiella domercqiae by BIOLOG analysis and routine yeast identification method. The crude biosurfactants produced from Y2A were obtained by extract with ethyl acetate and proved to be a mixture of glycolipids by thin layer chromatography (TLC). The main product was separated and purified by HPLC and then characterized as sophorolipid by nuclear magnetic resonance (NMR) and mass spectroscopy (MS). This is the first report on sophorolipids produced from W. domercqiae. Subsequently, the cytotoxic effects of the sophorolipid on cancer cells of H7402, A549, HL60 and K562 were investigated by MTT assay. The results showed a dose-dependent inhibition ratio on cell viability according to the drug concentration ≤62.5 μg/ml. These findings suggested that the sophorolipid produced by W. domercqiae have anticancer activity.

Keywords
Sophorolipid; Wickerhamiella domercqiae; Human cancer cell; Anticancer activity
First Page Preview
Production, structure elucidation and anticancer properties of sophorolipid from Wickerhamiella domercqiae
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 3, 3 July 2006, Pages 501–506
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering