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Kinetics of immobilized cyclodextrin gluconotransferase produced by Bacillus macerans ATCC 8244

Paper ID Volume ID Publish Year Pages File Format Full-Text
18451 42723 2006 4 PDF Available
Title
Kinetics of immobilized cyclodextrin gluconotransferase produced by Bacillus macerans ATCC 8244
Abstract

The extracellular enzyme cyclodextrin gluconotransferase (CGTase, EC 2.4.1.19) was produced by Bacillus macerans ATCC 8244 at optimized conditions. The CGTase activity was found to be 495 μg β-CD/(ml min). The enzyme was partially purified by 40–60% ammonium sulfate precipitation. The enzyme was immobilized by sodium alginate beads and kinetic studies were performed. The enzymatic activity of both free and immobilized CGTase was highest at temperature 60 °C and pH 7.5. The analysis of kinetic parameters Km and Vmax was obtained by the action of CGTase on the starch with respect to β-CD. The enzyme exhibits substrate inhibition and the values are 10 and 5.5 μg starch/ml for free and immobilized enzyme, respectively. The effect of salt concentration on enzyme activity was studied and it was found that Cu2+, Fe2+, Zn2+, Co2+, Mg2+ and Hg2+ were inhibitory, however Ca2+ showed a relative increase in enzyme activity. The immobilization of the enzyme showed a high operational stability by retaining almost 75% of the initial activity after seventh use.

Keywords
Cyclodextrin gluconotransferase (CGTase); Alginate; Immobilization; Kinetics
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Kinetics of immobilized cyclodextrin gluconotransferase produced by Bacillus macerans ATCC 8244
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 3, 3 July 2006, Pages 507–510
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us