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Chemical delivery microsystem for single-molecule analysis using multilaminar continuous flow

Paper ID Volume ID Publish Year Pages File Format Full-Text
18453 42723 2006 7 PDF Available
Title
Chemical delivery microsystem for single-molecule analysis using multilaminar continuous flow
Abstract

The fluidic operations, such as chemical delivery, hydrodynamic force control, solutions exchange, etc., to a desired location in a microfluidic channel are one of the most important technical aspects to progress single-molecule studies in microfluidic devices. This paper experimentally demonstrates multilaminar flow-based fluidic operations to supply and remove chemicals to an immobilized single-molecule on an inner surface of a microchannel to develop a future platform microfluidic device for single-molecule analysis. The microfluidic device, having multiple inlets and single outlet embedded with a micropump for each inlet, can generate continuous multiple laminar flow inside of the microchannel. The multiple laminar flow control capabilities were experimentally evaluated to deliver any single laminar flow to a desired location and to control flow speed to minimize hydrodynamic force to the target single-molecule. As a demonstration of single-molecule analysis using this format, ATP dependent rotation of F1-ATPase was investigated. As a result, the rotation of F1-ATPase was successfully investigated. This format showed many advantages and flexibility to realize the future platform of single-molecule studies.

Keywords
Chemical delivery; Microfluidics; Multilaminar flow; Single molecular analysis
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Chemical delivery microsystem for single-molecule analysis using multilaminar continuous flow
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 3, 3 July 2006, Pages 519–525
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us