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Induction of secondary metabolite production in transformed callus of Ammi majus L. grown after electromagnetic treatment of the culture medium

Paper ID Volume ID Publish Year Pages File Format Full-Text
18458 42724 2006 6 PDF Available
Title
Induction of secondary metabolite production in transformed callus of Ammi majus L. grown after electromagnetic treatment of the culture medium
Abstract

High-performance liquid chromatography analysis of crude chloroform and methanol extracts as well as methanol preparations subjected to enzymatic hydrolysis indicated two-fold higher accumulation of umbelliferone in transformed Ammi majus L. callus grown on medium exposed to ADR-4® (energy stimulator plate) in comparison to the control cultures. Elicitation with ADR-4® induced also two times higher accumulation of bergapten. Three different techniques: Soxhlet extraction, ultrasonication and accelerated solvent extraction (ASE) applied for the extraction of coumarins, furanocoumarins and corresponding glycosides from transformed callus of A. majus elicited by ADR-4® plates were compared. ASE is shown to be the most efficient extraction method.

Keywords
Accelerated solvent extraction (ASE); ADR-4® plates; Ammi majus; Bergapten; Magnetic field; Umbelliferone
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Induction of secondary metabolite production in transformed callus of Ammi majus L. grown after electromagnetic treatment of the culture medium
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 7, 3 November 2006, Pages 1386–1391
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us