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Purification and identification of a Bacillus nitroreductase: Potential use in 3,5-DNBTF biosensoring system

Paper ID Volume ID Publish Year Pages File Format Full-Text
18475 42724 2006 8 PDF Available
Title
Purification and identification of a Bacillus nitroreductase: Potential use in 3,5-DNBTF biosensoring system
Abstract

We have investigated a specific enzymatic biosensor for detecting target pollutant 3,5-dinitro-trifluoromethylbenzene (3,5-DNBTF). The predicted enzyme is a nitroreductase that catalyzes the total nitroreduction of 3,5-DNBTF to its corresponding diamine. The photo-activation of this diamine offers a large panel of detection tools. After broad screening of microorganisms, only the strains belonging to the genus Bacillus were able to reduce the two nitro groups of 3,5-DNBTF. Among them, Bacillus LMA, isolated from explosives-polluted effluents, was the most efficient in reducing this compound. The involved nitroreductase was identified by 2D gel electrophoresis coupled to mass spectrometry, as the Bacillus subtilis oxygen-insensitive nitroreductase NfrA. The enzyme was purified by mono-P chromatofocusing.

Keywords
Nitroreductase; Bacillus; Protein analysis; Nitroaromatic compounds; Biosensor
First Page Preview
Purification and identification of a Bacillus nitroreductase: Potential use in 3,5-DNBTF biosensoring system
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 7, 3 November 2006, Pages 1499–1506
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering