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Study on synthesis of a hydrophilic bead carrier containing epoxy groups and its properties for glucoamylase immobilization

Paper ID Volume ID Publish Year Pages File Format Full-Text
18484 42725 2006 8 PDF Available
Title
Study on synthesis of a hydrophilic bead carrier containing epoxy groups and its properties for glucoamylase immobilization
Abstract

A series of macroporous, hydrophilic and reactive bead carriers containing epoxy groups were synthesized by suspension polymerization of glycidyl methacrylate (GMA) and methacrylic acid (MAA), using divinyl benzene (DVB) as a crosslinking agent, while a mixture of linear poly(vinyl acetate) (PVAc) and toluene were used as pore-forming agents, meanwhile, the resulting carriers were employed in the immobilization of glucoamylase, i.e. glucoamylase be covalently immobilized because of the excellent reactivity of the epoxy groups in the carriers. The activity recovery of the immobilized glucoamylase could reach to 78%, the apparent Km value of the immobilized glucoamylase was about three times than that of the free ones, and the apparent Vmax value of the immobilized glucoamylase was slightly larger than that of the latter. For the hydrolysis of soluble starch using the resulting immobilized glucoamylase, the optimal pH and temperature were 5.0–6.0 and 55 °C, respectively. Otherwise, the immobilized glucoamylase exhibits excellent thermal stability, reusability and endurance property for acid and alkali.

Keywords
Bead carriers; Immobilized glucoamylase; Macroporous carrier; Suspension polymerization
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Study on synthesis of a hydrophilic bead carrier containing epoxy groups and its properties for glucoamylase immobilization
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 4, 2 August 2006, Pages 540–547
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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Any Questions? feel free to contact us