fulltext.study @t Gmail

Growth and differentiation of osteoblastic cells on 13–93 bioactive glass fibers and scaffolds

Paper ID Volume ID Publish Year Pages File Format Full-Text
1849 92 2008 10 PDF Available
Title
Growth and differentiation of osteoblastic cells on 13–93 bioactive glass fibers and scaffolds
Abstract

This in vitro study was conducted to evaluate the ability of two types of constructs of bioactive, silica-based 13–93 glass fibers to support the growth and differentiation of MC3T3-E1 osteoblastic cells. The two types of constructs tested included single-layer 13–93 glass fiber rafts and three-dimensional porous scaffolds formed from sintered 13–93 fibers. Scanning electron micrographs showed a closely adhering, well-spread morphology of MC3T3-E1 cells seeded on both types of constructs. The scanning electron microscopy images also showed a continuous increase in cell densities during a 6 day incubation on 13–93 glass fiber rafts and scaffolds. Quantitative fluorescence measurements of DNA also revealed a linear increase in cell density during a 6 day incubation on both types of 13–93 constructs. Examination of scaffolds incubated in MTT containing medium showed the presence of metabolically active viable cells within the interior of the scaffold. The addition of ascorbic acid to MC3T3-E1 cells cultured on the 13–93 glass fibers triggered a threefold increase in alkaline phosphatase, a key indicator of osteoblast differentiation. The sintered scaffolds were found to have open, interconnected pores favorable for tissue ingrowth with a compressive strength similar to cancellous bone. Collectively, the results indicate that 13–93 glass fiber scaffolds are a favorable substrate for the growth and differentiation of osteoblasts and a promising material for bone tissue engineering and repair of bone defects.

Keywords
Glass fibers; Scaffolds; Osteoblasts; Proliferation; Alkaline phosphatase
First Page Preview
Growth and differentiation of osteoblastic cells on 13–93 bioactive glass fibers and scaffolds
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 4, Issue 2, March 2008, Pages 387–396
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us