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Optimization of glucose-6-phosphate dehydrogenase releasing from Candida guilliermondii by disruption with glass beads

Paper ID Volume ID Publish Year Pages File Format Full-Text
18492 42725 2006 5 PDF Available
Title
Optimization of glucose-6-phosphate dehydrogenase releasing from Candida guilliermondii by disruption with glass beads
Abstract

The release of glucose-6-phosphate dehydrogenase (G6PD) from Candida guilliermondii, grown on rice straw hydrolysate, by vortex agitation with glass beads was studied. The effects of cell suspension concentration and disruption time on volumetric activity, total protein and specific activity were evaluated from a 22 full-factorial central composite design. By using the response surface methodology, a second-order model was proposed to represent enzyme specific activity as a function of disruption time (X1) and cell suspension concentration (X2). Under optimum conditions (X1 = 5 min and X2 = 11.5 g l−1 the model predicted a G6PD specific activity of 0.88 U mg−1.

Keywords
Candida guilliermondii; Cell disruption; Glass beads; Glucose-6-phosphate dehydrogenase; Response-surface methodology
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 4, 2 August 2006, Pages 591–595
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us