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Immobilization of bovine catalase in sol–gels

Paper ID Volume ID Publish Year Pages File Format Full-Text
18498 42725 2006 8 PDF Available
Title
Immobilization of bovine catalase in sol–gels
Abstract

Enzyme immobilization offers numerous advantages in terms of practical applications spanning biosensors to reactors. Bovine liver catalase (BLC [EC 1.11.1.6.]) was immobilized in tetraethoxyorthosilicate (TEOS) based sol–gels. TEOS sol–gels were found to be suitable for entrapping BLC with a high degree of immobilization. Kinetic constants for the catalatic mode of action of the immobilized enzyme were determined. Immobilized BLC retained less than 1% catalatic but 50% peroxidatic activity compared to its free counterpart. Addition of various additives did not improve the catalatic performance. Structural integrity of the immobilized enzyme and availability of active sites was confirmed by UV–vis-spectroscopy suggesting that the relatively low catalatic activity is due to effects of the sol–gel matrix on oxygen yielding reactions and dissolution into aqueous media. These data also imply that there may be significant mass transfer resistance due to the matrix depending on the nature of the substrate and product. Spectroscopic studies also indicated that the sol–gel matrix protects the catalase from formation of the inactive compound II. Heat inactivation studies showed a significantly improved thermostability of the immobilized preparation with a doubling of the inactivation energy when compared to the free enzyme. Sol–gel immobilized BLC was found to be stable for 17 consecutive reactor batch cycles with no apparent loss in activity.

Keywords
Catalase; Hydrogen peroxide; Immobilized enzyme; Sol–gel; Thermostability
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Immobilization of bovine catalase in sol–gels
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 4, 2 August 2006, Pages 626–633
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us