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Thermostable α-amylase production by Bacillus subtilis entrapped in calcium alginate gel capsules

Paper ID Volume ID Publish Year Pages File Format Full-Text
18507 42725 2006 7 PDF Available
Title
Thermostable α-amylase production by Bacillus subtilis entrapped in calcium alginate gel capsules
Abstract

Bacillus subtilis cells were immobilized by entrapment in calcium alginate gel capsules and the immobilized biocatalyst was used for the semi-continuous production of α-amylase. The α-amylase yield and operational stability of the immobilized system were increased by tailoring the capsules’ characteristics. Capsules prepared from 2% (w/v) sodium alginate and 3.5% (w/v) CaCl2 were the best support for cell immobilization, providing 2.5-fold higher α-amylase production in comparison to the freely suspended cells. Immobilized biocatalysts sustained 90% of their initial productivity over five sequential batches in a 10-day period, while amylase production by free cells declined sharply after the second use. Even higher operational stability was achieved when the capsules were treated with 2% (w/v) CaCl2 for 30 min before each batch. In this case the appearance of free cells in the medium was minimized and the immobilized system retained more than 85% of its initial efficiency after 15 fermentation batches, producing more than 1,450,000 units of extracellular α-amylase during this period.

Keywords
α-Amylase; Production; Immobilization; Alginate; Capsules
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Thermostable α-amylase production by Bacillus subtilis entrapped in calcium alginate gel capsules
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 4, 2 August 2006, Pages 690–696
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us