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Alkaline protease production from alkalophilic Bacillus sp. isolated from natural habitats ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
18509 42725 2006 8 PDF Available
Title
Alkaline protease production from alkalophilic Bacillus sp. isolated from natural habitats ☆
Abstract

Bacillus strains isolated under extreme alkaline conditions (Izmir, Turkey), were screened and identified for high alkaline protease activity. Strains with high protease yields were optimized with respect to inoculum concentration, temperature, agitation speed, initial medium pH and incubation time. Three Bacillus strains coded as I18, L18 and L21 showed high potential, for alkaline protease activity (160–222 U/ml) among 85 isolates. The specific growth rates were estimated from the growth curves as 0.49 h−1 for I18, as 0.6 and 0.7 h−1 for L18 and L21, respectively. The optimum temperatures were determined as 30 °C for strain I18 and 37 °C for the strains L18 and L21. Similarly, the optimum agitation speeds were 100 rpm for I18 and 180 rpm for L18 and L21. For all three strains, the optimum inoculation ratio and incubation time, were determined as 5% (v/v) and 96 h, respectively. The optimum initial media pH was found as pH 10 for strain L18 and L21. Bacillus sp. L21 with the highest specific protease activity (60 U/mg protein) and a broader pH range was chosen for further study. The biomass and product yield for this strain was determined as 0.023 g cell/g glucose and 0.021 U/g glucose, respectively. The crude enzyme of this strain was further characterized and was determined as a bleach stable, serine alkaline protease with an optimum temperature of 60 °C and a pH of 11, with a potential to be a candidate for the applications in the detergent industry.

Keywords
Alkaline protease; Bacillus sp.; Microbial enzymes; Enzyme production
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Alkaline protease production from alkalophilic Bacillus sp. isolated from natural habitats ☆
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 4, 2 August 2006, Pages 703–710
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us