fulltext.study @t Gmail

Production of mono-olein by immobilized Staphylococcus simulans lipase in a solvent-free system: Optimization by response surface methodology

Paper ID Volume ID Publish Year Pages File Format Full-Text
18511 42725 2006 7 PDF Available
Title
Production of mono-olein by immobilized Staphylococcus simulans lipase in a solvent-free system: Optimization by response surface methodology
Abstract

Mono-olein is the most widely used emulsifier in food and pharmaceutical industries. It was produced by direct esterification of oleic acid with glycerol using immobilized Staphylococcus simulans lipase. The best mono-olein yield was achieved with CaCO3 as support. No organic solvent was necessary to solubilize the substrates. This allowed the use of a reaction medium solely composed of the necessary substrates. Response surface methodology based on three-level, three-variable central composite rotatable designs was employed to optimize three reaction variables: the amount of lipase, the initial added water and the oleic acid to glycerol molar ratio. The optimum conditions predicted for achieving 70.6% yield of mono-olein were as follows: an amount of lipase of 100 IU, an initial added water of 5% (w/w) and an oleic acid to glycerol molar ratio of 0.2. The experimental results confirmed the predicted values.

Keywords
MGs, monoglycerides; MO, mono-olein; DO, diolein; OA, oleic acid; TO, triolein; SSL, Staphylococcus simulans lipase; Lyo, lyophilized; i, immobilized; IU, international unit; TLC, thin layer chromatography; RSM, response surface methodologyMono-olein; Sol
First Page Preview
Production of mono-olein by immobilized Staphylococcus simulans lipase in a solvent-free system: Optimization by response surface methodology
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 4, 2 August 2006, Pages 717–723
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us