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Isolation, purification and characterization of the xylanase produced by Arthrobacter sp. MTCC 5214 when grown in solid-state fermentation

Paper ID Volume ID Publish Year Pages File Format Full-Text
18513 42725 2006 11 PDF Available
Title
Isolation, purification and characterization of the xylanase produced by Arthrobacter sp. MTCC 5214 when grown in solid-state fermentation
Abstract

Thermoalkalophilic Arthrobacter sp. produced extracellular xylanase, when wheat bran, rice husk, rice bran and bagassae were used as carbon source under solid-state fermentation (SSF). The xylanase enzyme was isolated by ammonium sulfate (80%) fractionation, and purified to homogeneity using size exclusion and ion exchange chromatography. The molecular mass of xylanase was ∼20 kDa. Enzyme retained 100% activity at pH 7 and 8 for 24 h. It was interesting to note that at higher pH such as 9, 10 and 11 the enzyme activity increased over the period of incubation. The optimum temperature for the enzyme activity was 100 °C at pH 9.0. At 80 °C and pH 9, half-life of enzyme was 30 min. Half-life of enzyme at 70 and 60 °C was 18 and 24 h, respectively. While at 50 °C the enzyme retained 79% of activity even after 48 h. For xylan, the enzyme gave a Km value of 0.9 mg/ml, and Vmax value of 3571 μmol/min/mg when the reaction was carried out at 100 °C and pH 9. In the presence of metal ions such as Co2+, Zn2+, Fe2+, Cu2+, Mg2+ and Ca2+ and metal chelator EDTA the activity of the enzyme increased. Whereas strong inhibition of the enzyme activity was observed in the presence of Hg2+. These are some novel characteristics that make this enzyme potentially very effective for industrial applications.

Keywords
Arthrobacter sp.; Wheat bran; Xylanase; Purification; Fermentation
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Isolation, purification and characterization of the xylanase produced by Arthrobacter sp. MTCC 5214 when grown in solid-state fermentation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 4, 2 August 2006, Pages 732–742
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us