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Thiobacillus thioparus immobilized by magnetic porous beads: Preparation and characteristic

Paper ID Volume ID Publish Year Pages File Format Full-Text
18518 42725 2006 8 PDF Available
Title
Thiobacillus thioparus immobilized by magnetic porous beads: Preparation and characteristic
Abstract

Novel Fe3O4/poly(styrene (St)–acrylic acid (AA)) magnetic porous beads (MPBs) were fabricated by a seed swelling process using magnetite particles as seeds. The porous beads were characterized with scanning electron microscopy (SEM). Magnetic properties of the MPBs were obtained by electron spin resonance (ESR) and vibrating-sample magnetometer (VSM). All the observations indicated that the beads were ferromagnetic. Thiobacillus thioparus was immobilized to the porous carrier at up to 9.3 × 109 cells/g MPBs. Compared with active carbon (AC) and agar as carriers, MPB as a carrier of immobilized Thiobacillus cells is the most efficient in removal of sulfide. Effects of the size of MPBs and surface carboxyl content on cell immobilization efficiency were investigated and the optimal pH and the temperature for the immobilized cells were determined. T. thioparus immobilized on MPB had a good storage stability. Continuous operation of a magnetically stabilized fluidized bed (MSFB) filled with MPB-immobilized T. thioparus at a flow rate of 160 ml/min resulted in the sulfide oxidizing rate of 8.64 kg S m−3 d−1.

Keywords
Magnetic porous beads; Thiobacillus thioparus; Magnetic stabilized fluidized bed reactor; Immobilization; Sulfide oxidation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 4, 2 August 2006, Pages 770–777
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us