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Production of itaconic acid by Pseudozyma antarctica NRRL Y-7808 under nitrogen-limited growth conditions

Paper ID Volume ID Publish Year Pages File Format Full-Text
18527 42725 2006 4 PDF Available
Title
Production of itaconic acid by Pseudozyma antarctica NRRL Y-7808 under nitrogen-limited growth conditions
Abstract

Pseudozyma antarctica NRRL Y-7808 was found to produce itaconic acid from glucose and other sugars under nitrogen-limited growth conditions. Other Pseudozyma strains screened, including a second strain of P. antarctica, did not produce this product; therefore itaconic acid production is not a common trait of the genus. Glucose and fructose were the most efficiently utilized substrates, followed by sucrose and maltose; lactose and glycerol were the poorest substrates. The maximum yield in flask experiments was 37.5% (approximately 30 g/L itaconic acid from 80 g/L glucose). The maximum rate of production in flask cultures was 248 mg L−1 h−1 at a C/N ratio of 116, while the best combination of rate and yield was produced at a C/N ratio of 175 (230 mg L−1 h−1 and 29%, respectively). A stirred-tank reactor process study resulted in a 20% yield and volumetric production rate of 110 mg L−1 h−1.

Keywords
Itaconic acid; Nitrogen limitation; Pseudozyma antarctica NRRL Y-7808; Sugar utilization; Bioreactor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 4, 2 August 2006, Pages 824–827
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us