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Performance of free Candida antarctica lipase B in the enantioselective esterification of (R)-ketoprofen

Paper ID Volume ID Publish Year Pages File Format Full-Text
18542 42725 2006 6 PDF Available
Title
Performance of free Candida antarctica lipase B in the enantioselective esterification of (R)-ketoprofen
Abstract

The performance of free Candida antarctica lipase B (CALB) in a mixed solvent system for enantioselective esterification of (R)-ketoprofen, leaving the target product (S)-ketoprofen in unreacted form was investigated. The effects of enzyme loading, substrate concentration, alcohol to acid molar ratio, type of solvents and reaction temperature were studied. The optimum reaction conditions obtained were 200 mg/ml of CALB and racemic ketoprofen concentration of 30 mM, with 20% (v/v) 1,2-dichloropropane–80% (v/v) n-hexane as solvent, alcohol to acid molar ratio of 1:1 at 40 °C. The conversion of (R)-ketoprofen, XR obtained was 81% with enantioselectivity (E) value of 10, enantiomeric excess of product (eep) of 66.4% and enantiomeric excess of substrate (ees) of 59.4% for the reaction time of 24 h.

Keywords
Candida antarctica lipase B; Ketoprofen; Enzymatic esterification; Enantiomer; Enantiopure
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Performance of free Candida antarctica lipase B in the enantioselective esterification of (R)-ketoprofen
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 4, 2 August 2006, Pages 924–929
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us