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Immobilization of polyphenol oxidase in conducting graft copolymers and determination of phenolic amount in red wines with enzyme electrodes

Paper ID Volume ID Publish Year Pages File Format Full-Text
18545 42725 2006 4 PDF Available
Title
Immobilization of polyphenol oxidase in conducting graft copolymers and determination of phenolic amount in red wines with enzyme electrodes
Abstract

Polyphenol oxidase was immobilized in several conducting copolymer matrices. Three different types of poly(methyl methacrylate-co-methyl thienyl methacrylate) matrices were used to obtain copolymers. Immobilization of enzyme was carried out by the entrapment of the enzyme in conducting copolymer matrices during electrochemical polymerization of pyrrole through thiophene moieties of the polymers. Maximum reaction rates, Michaelis–Menten constants, optimum temperature and pH values and operational stabilities of the enzyme electrodes were investigated. Total amount of phenolic compounds in some Turkish red wines was analyzed using these electrodes.

Keywords
Polyphenol oxidase; Immobilization; Electrochemistry; Polyphenols; Red wines
First Page Preview
Immobilization of polyphenol oxidase in conducting graft copolymers and determination of phenolic amount in red wines with enzyme electrodes
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 4, 2 August 2006, Pages 945–948
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering