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Immobilization of chitosan-modified invertase on alginate-coated chitin support via polyelectrolyte complex formation

Paper ID Volume ID Publish Year Pages File Format Full-Text
18554 42726 2006 6 PDF Available
Title
Immobilization of chitosan-modified invertase on alginate-coated chitin support via polyelectrolyte complex formation
Abstract

Saccharomyces cerevisiae invertase was chemically modified with chitosan and further immobilized on sodium alginate-coated chitin support. The yield of immobilized protein was determined as 85% and the enzyme retained 97% of the initial chitosan-invertase activity. The optimum temperature for invertase was increased by 10 °C and its thermostability was enhanced by about 9 °C after immobilization. The immobilized enzyme was stable against incubation in high ionic strength solutions and was four-fold more resistant to thermal treatment at 65 °C than the native counterpart. The biocatalyst prepared retained 80% of the original catalytic activity after 50 h under continuous operational regime in a packed bed reactor.

Keywords
Invertase; Chitin; Enzyme immobilization; Enzyme stability; Polyelectrolyte complex
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Immobilization of chitosan-modified invertase on alginate-coated chitin support via polyelectrolyte complex formation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 38, Issues 1–2, 3 January 2006, Pages 22–27
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us