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Upgrading of an industrial lignin by using laccase produced by Fusarium proliferatum and different laccase-mediator systems

Paper ID Volume ID Publish Year Pages File Format Full-Text
18557 42726 2006 9 PDF Available
Title
Upgrading of an industrial lignin by using laccase produced by Fusarium proliferatum and different laccase-mediator systems
Abstract

An industrial lignin recovered from a paper factory effluent was characterized and treated in buffered media under three different conditions. The first system contained only partially purified laccase produced by Fusarium proliferatum. The other two were supplemented with 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) or 1-hydroxylbenzotriazole (1-HBT) as mediators. During incubation the lignin remained solubilized and its spectral and chromatographic characteristics depended on time and conditions. UV–vis spectrum of lignin recovered from the first system showed a strong increase at the outset of incubation, reflecting the introduction of new functional groups in the polymer. No changes in its molecular mass distribution pattern were detected. Longer incubation produced minor alterations in its UV–vis spectrum, together with a polymerization in the recovered substrate, appearing as a new peak around 200 kDa. Lignin recovered from the laccase-ABTS system showed an intensive depolymerization together with a minor polymerization also in the high molecular mass fraction (200 kDa). That lignin sample was soluble under acid conditions. These sharp modifications contrasted with those detected in the lignin incubated with 1-HBT as mediator.Treating industrial lignin by these simple procedures rapidly enhances the technological potential of this paper-mill byproduct.

Keywords
Laccase; UV–vis spectrum; Fusarium proliferatum
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Upgrading of an industrial lignin by using laccase produced by Fusarium proliferatum and different laccase-mediator systems
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 38, Issues 1–2, 3 January 2006, Pages 40–48
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us