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Production of an oxidant and SDS-stable alkaline protease from an alkaophilic Bacillus clausii I-52 by submerged fermentation: Feasibility as a laundry detergent additive

Paper ID Volume ID Publish Year Pages File Format Full-Text
18576 42726 2006 8 PDF Available
Title
Production of an oxidant and SDS-stable alkaline protease from an alkaophilic Bacillus clausii I-52 by submerged fermentation: Feasibility as a laundry detergent additive
Abstract

An investigation was carried out on enzyme production of an oxidant and SDS-stable alkaline protease secreted by Bacillus clausii I-52 using the submerged fermentation and its application as a detergent additive. Maximum enzyme activity was produced when cells were grown under the submerged fermentation conditions at 37 °C for 48 h with an aeration rate of 1.5 vvm and agitation rate of 700 rpm in a medium (pH 10.65) containing (w/v): soybean meal, 20; wheat flour, 10; liquid maltose, 25; K2HPO4, 4; Na2HPO4, 1; MgSO4·7H2O, 0.1; NaCl, 4; FeSO4·7H2O, 0.5; Na2CO3, 6. The alkaline protease produced was found to be highly compatible and stable against not only the commercial detergent components such as α-orephin sulfonate and zeolite but also the commercial detergent preparations. Wash performance analysis using EMPA test fabrics revealed that BCAP exhibited high efficiency for the removal of protein stains in the presence of commercial detergents as well as surfactants. These results suggest that the alkaline protease produced from B. clausii I-52 which showed high stability against detergents has significance for an industrial perspective, especially, detergent additive.

Keywords
AOS, α-orephin sulfonate; BCAP, Bacillus clausii alkaline protease; cdw, cell dry weight; DO, dissolved oxygen; LAS, linear alkylbenzene sulfonates; OD, optical density; PG, propylene glycol; SDS, sodium dodecyl sulphate; SmF, submerged fermentation; TSB,
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Production of an oxidant and SDS-stable alkaline protease from an alkaophilic Bacillus clausii I-52 by submerged fermentation: Feasibility as a laundry detergent additive
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 38, Issues 1–2, 3 January 2006, Pages 176–183
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us