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PVA-gel (Lentikats®) as an effective matrix for yeast strain immobilization aimed at heterologous protein production

Paper ID Volume ID Publish Year Pages File Format Full-Text
18577 42726 2006 6 PDF Available
Title
PVA-gel (Lentikats®) as an effective matrix for yeast strain immobilization aimed at heterologous protein production
Abstract

Three different yeast strains, namely Saccharomyces cerevisiae mnn1mnn9, Kluyveromyces lactis JA6/GAA and Zygosaccharomyces bailii [pZ3klIL-1β], were entrapped in polyvinyl alcohol (PVA) gel particles, obtained following the commercially available immobilization kit named Lentikat®. After immobilization in the PVA-gel particles, yeast cells remained viable: colonization of the gel matrix reached up 100 mg d.w. of cells cm−3 gel for all the strains examined.Lentikats® of K. lactis JA6/GAA and Z. bailii [pZ3klIL-1β] showed to be suitable for the continuous production of glucoamylase and interleukin 1β, respectively, when employed under non-selective conditions. They were of easy handiness and showed excellent mechanical properties during prolonged operation in stirred tank reactors.

Keywords
Immobilization; Polyvinyl alcohol; Yeast; Heterologous proteins
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PVA-gel (Lentikats®) as an effective matrix for yeast strain immobilization aimed at heterologous protein production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 38, Issues 1–2, 3 January 2006, Pages 184–189
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us