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Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate

Paper ID Volume ID Publish Year Pages File Format Full-Text
18581 42726 2006 5 PDF Available
Title
Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate
Abstract

Chitosanolytic activity was monitored using chitosan impregnated with Remazol Brilliant Blue R dye (RBB). Low-yielding chitosan (1%, w/v) hydrolysis was modelled using Viscozyme® L (50%, w/w), a commercial mixed-glucanase, and was found to perform optimally at pH 4.5 and 50 °C. The chitosanase assay was performed on powdered (RBB-P) and colloidal form (RBB-C) of the dyed chitosan. Hydrolysis of chitosan was accompanied by the release of water-soluble dyed-hydrolysate, which can be monitored rapidly using microplate reader at 595 nm. The result obtained from this method was in agreement with the determination of reducing end by using the traditional, complex and time-consuming DNS method. The method is significantly rapid and more sensitive than DNS method.

Keywords
Chitosanase; RBB-chitosan; Rapid method
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Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 38, Issues 1–2, 3 January 2006, Pages 215–219
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us