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Porcine bone marrow stromal cell differentiation on heparin-adsorbed poly(e-caprolactone)–tricalcium phosphate–collagen scaffolds

Paper ID Volume ID Publish Year Pages File Format Full-Text
1859 93 2009 11 PDF Available
Title
Porcine bone marrow stromal cell differentiation on heparin-adsorbed poly(e-caprolactone)–tricalcium phosphate–collagen scaffolds
Abstract

We evaluate the potential of heparin as a substrate component for the fabrication of bone tissue engineering constructs using poly(e-caprolactone)–tricalcium phosphate–collagen type I (PCL–TCP–Col) three-dimensional (3-D) scaffolds. First we explored the ability of porcine bone marrow precursor cells (MPCs) to differentiate down both the adipogenic and osteogenic pathways within 2-D culture systems, with positive results confirmed by Oil-Red-O and Alizarin Red staining, respectively. Secondly, we examined the influence of heparin on the interaction and behaviour of MPCs when seeded onto PCL–TCP–Col 3-D scaffolds, followed by their induction into the osteogenic lineage. Our 3-D findings suggest that cell metabolism and proliferation increased between days 1 and 14, with deposition of extracellular matrix also observed up to 28 days. However, no noticeable difference could be detected in the extent of osteogenesis for PCL–TCP–Col scaffolds groups with the addition of heparin compared to identical control scaffolds without the addition of heparin.

Keywords
Scaffold; PCL–Collagen; Cell differentiation; Heparin
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Porcine bone marrow stromal cell differentiation on heparin-adsorbed poly(e-caprolactone)–tricalcium phosphate–collagen scaffolds
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 5, Issue 9, November 2009, Pages 3305–3315
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us