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Enzymatic synthesis of polyglucosylfructosides from sucrose alone by a novel α-glucosidase isolated from the digestive juice of Archachatina ventricosa (Achatinideae)

Paper ID Volume ID Publish Year Pages File Format Full-Text
18601 42727 2007 8 PDF Available
Title
Enzymatic synthesis of polyglucosylfructosides from sucrose alone by a novel α-glucosidase isolated from the digestive juice of Archachatina ventricosa (Achatinideae)
Abstract

A novel α-glucosidase was purified from the digestive juice of the snail Archachatina ventricosa using Q Sepharose Fast Flow ion-exchange chromatography and preparative polyacrylamide gel electrophoresis. The enzyme has a molecular weight of 138 kDa by gel filtration and 66 kDa on SDS-PAGE, which corresponds to a homodimeric structure. The pH and temperature optima of sucrose hydrolytic activity are 5.5 and 45 °C, respectively. The enzyme is active on sucrose, pNP α-d-glucopyranoside, maltose and is unable to hydrolyse raffinose, cellobiose and polymers such as amylose, inulin and cellulose. We investigate the transglycosylation potential of this enzyme using sucrose as single glucosyle donor and acceptor substrate in order to access specific features which are of interest from a biotechnological point of view (i.e. oligosaccharides synthesis). The enzyme (1 U/ml of hydrolytic activity) incubated with sucrose (1.5 M) exhibits transglycosylation activity producing polyglucosylfructosides and disaccharides in lesser amount. About 60% of the initial sucrose was consumed after 72 h of incubation at 35 °C.

Keywords
Snail (Archachatina ventricosa); Digestive juice; α-Glucosidase; Purification; Sucrose; Transglycosylation; Polyglucosylfructosides; NMR spectroscopy
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Enzymatic synthesis of polyglucosylfructosides from sucrose alone by a novel α-glucosidase isolated from the digestive juice of Archachatina ventricosa (Achatinideae)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 42, Issue 1, 3 December 2007, Pages 44–51
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us